SARS-coronavirus replication in human peripheral monocytes/macrophages.
Identifieur interne : 004651 ( Main/Exploration ); précédent : 004650; suivant : 004652SARS-coronavirus replication in human peripheral monocytes/macrophages.
Auteurs : Mamadi Yilla [États-Unis] ; Brian H. Harcourt ; Carole J. Hickman ; Marcia Mcgrew ; Azaibi Tamin ; Cynthia S. Goldsmith ; William J. Bellini ; Larry J. AndersonSource :
- Virus research [ 0168-1702 ] ; 2005.
Descripteurs français
- KwdFr :
- Adulte, Anticorps antiviraux, Humains, Interféron alpha (biosynthèse), Macrophages (ultrastructure), Macrophages (virologie), Microscopie électronique, Monocytes (ultrastructure), Monocytes (virologie), Phagocytose, Réplication virale, Syndrome respiratoire aigu sévère (virologie), Techniques in vitro, Virus du SRAS (immunologie), Virus du SRAS (pathogénicité), Virus du SRAS (physiologie), Virus du SRAS (ultrastructure).
- MESH :
- biosynthèse : Interféron alpha.
- immunologie : Virus du SRAS.
- pathogénicité : Virus du SRAS.
- physiologie : Virus du SRAS.
- virologie : Macrophages, Monocytes, Syndrome respiratoire aigu sévère.
- Adulte, Anticorps antiviraux, Humains, Macrophages, Microscopie électronique, Monocytes, Phagocytose, Réplication virale, Techniques in vitro, Virus du SRAS.
English descriptors
- KwdEn :
- Adult, Antibodies, Viral, Humans, In Vitro Techniques, Interferon-alpha (biosynthesis), Macrophages (ultrastructure), Macrophages (virology), Microscopy, Electron, Monocytes (ultrastructure), Monocytes (virology), Phagocytosis, SARS Virus (immunology), SARS Virus (pathogenicity), SARS Virus (physiology), SARS Virus (ultrastructure), Severe Acute Respiratory Syndrome (virology), Virus Replication.
- MESH :
- chemical , biosynthesis : Interferon-alpha.
- chemical : Antibodies, Viral.
- immunology : SARS Virus.
- pathogenicity : SARS Virus.
- physiology : SARS Virus.
- ultrastructure : Macrophages, Monocytes, SARS Virus.
- virology : Macrophages, Monocytes, Severe Acute Respiratory Syndrome.
- Adult, Humans, In Vitro Techniques, Microscopy, Electron, Phagocytosis, Virus Replication.
Abstract
A novel coronavirus (CoV) has been described in association with cases of severe acute respiratory syndrome (SARS). The virus, SARS-CoV, differs from the previously described human coronaviruses, 229E and OC43. 229E was previously shown to productively infect human monocytes/macrophages, whereas OC43 poorly infected the cells. In this study, we examined whether SARS-CoV could productively infect purified monocytes/macrophages (PM) derived from human donor cells. Unlike 229E-infected cells, which produced viral titers of 10(3.5) to 10(6)TCID50/ml, SARS-CoV replicated poorly in PM, producing titers of 10(1.75) to 10(2)TCID50/ml. This finding was similar to results reported for OC43-infected cells, with titers ranging from 10(1.2) to 10(2.7)TCID50/ml. Of interest, SARS-CoV proteins were detected only in PM that did not produce significant amounts of interferon (IFN)-alpha, and in one such case, preliminary electron microscope studies demonstrated that SARS-CoV-like particles could enter the cells, possibly via phagocytosis. These results suggest that SARS-CoV, like human CoV OC43, poorly infects human PM, and production of IFN-alpha by these cells further limits the infection. Given the importance of monocytes/macrophages to the immune response, it is possible that their infection by SARS-CoV and alteration of this infection by IFN-alpha may be important to the course of the infection in humans.
DOI: 10.1016/j.virusres.2004.09.004
PubMed: 15567038
Affiliations:
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Harcourt</name></author><author><name sortKey="Hickman, Carole J" sort="Hickman, Carole J" uniqKey="Hickman C" first="Carole J" last="Hickman">Carole J. Hickman</name></author><author><name sortKey="Mcgrew, Marcia" sort="Mcgrew, Marcia" uniqKey="Mcgrew M" first="Marcia" last="Mcgrew">Marcia Mcgrew</name></author><author><name sortKey="Tamin, Azaibi" sort="Tamin, Azaibi" uniqKey="Tamin A" first="Azaibi" last="Tamin">Azaibi Tamin</name></author><author><name sortKey="Goldsmith, Cynthia S" sort="Goldsmith, Cynthia S" uniqKey="Goldsmith C" first="Cynthia S" last="Goldsmith">Cynthia S. Goldsmith</name></author><author><name sortKey="Bellini, William J" sort="Bellini, William J" uniqKey="Bellini W" first="William J" last="Bellini">William J. Bellini</name></author><author><name sortKey="Anderson, Larry J" sort="Anderson, Larry J" uniqKey="Anderson L" first="Larry J" last="Anderson">Larry J. Anderson</name></author></analytic><series><title level="j">Virus research</title><idno type="ISSN">0168-1702</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Antibodies, Viral</term><term>Humans</term><term>In Vitro Techniques</term><term>Interferon-alpha (biosynthesis)</term><term>Macrophages (ultrastructure)</term><term>Macrophages (virology)</term><term>Microscopy, Electron</term><term>Monocytes (ultrastructure)</term><term>Monocytes (virology)</term><term>Phagocytosis</term><term>SARS Virus (immunology)</term><term>SARS Virus (pathogenicity)</term><term>SARS Virus (physiology)</term><term>SARS Virus (ultrastructure)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term><term>Anticorps antiviraux</term><term>Humains</term><term>Interféron alpha (biosynthèse)</term><term>Macrophages (ultrastructure)</term><term>Macrophages (virologie)</term><term>Microscopie électronique</term><term>Monocytes (ultrastructure)</term><term>Monocytes (virologie)</term><term>Phagocytose</term><term>Réplication virale</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Techniques in vitro</term><term>Virus du SRAS (immunologie)</term><term>Virus du SRAS (pathogénicité)</term><term>Virus du SRAS (physiologie)</term><term>Virus du SRAS (ultrastructure)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Interferon-alpha</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Interféron alpha</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Macrophages</term><term>Monocytes</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Macrophages</term><term>Monocytes</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Macrophages</term><term>Monocytes</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Humans</term><term>In Vitro Techniques</term><term>Microscopy, Electron</term><term>Phagocytosis</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Anticorps antiviraux</term><term>Humains</term><term>Macrophages</term><term>Microscopie électronique</term><term>Monocytes</term><term>Phagocytose</term><term>Réplication virale</term><term>Techniques in vitro</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A novel coronavirus (CoV) has been described in association with cases of severe acute respiratory syndrome (SARS). The virus, SARS-CoV, differs from the previously described human coronaviruses, 229E and OC43. 229E was previously shown to productively infect human monocytes/macrophages, whereas OC43 poorly infected the cells. In this study, we examined whether SARS-CoV could productively infect purified monocytes/macrophages (PM) derived from human donor cells. Unlike 229E-infected cells, which produced viral titers of 10(3.5) to 10(6)TCID50/ml, SARS-CoV replicated poorly in PM, producing titers of 10(1.75) to 10(2)TCID50/ml. This finding was similar to results reported for OC43-infected cells, with titers ranging from 10(1.2) to 10(2.7)TCID50/ml. Of interest, SARS-CoV proteins were detected only in PM that did not produce significant amounts of interferon (IFN)-alpha, and in one such case, preliminary electron microscope studies demonstrated that SARS-CoV-like particles could enter the cells, possibly via phagocytosis. These results suggest that SARS-CoV, like human CoV OC43, poorly infects human PM, and production of IFN-alpha by these cells further limits the infection. Given the importance of monocytes/macrophages to the immune response, it is possible that their infection by SARS-CoV and alteration of this infection by IFN-alpha may be important to the course of the infection in humans.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Géorgie (États-Unis)</li></region></list><tree><noCountry><name sortKey="Anderson, Larry J" sort="Anderson, Larry J" uniqKey="Anderson L" first="Larry J" last="Anderson">Larry J. Anderson</name><name sortKey="Bellini, William J" sort="Bellini, William J" uniqKey="Bellini W" first="William J" last="Bellini">William J. Bellini</name><name sortKey="Goldsmith, Cynthia S" sort="Goldsmith, Cynthia S" uniqKey="Goldsmith C" first="Cynthia S" last="Goldsmith">Cynthia S. Goldsmith</name><name sortKey="Harcourt, Brian H" sort="Harcourt, Brian H" uniqKey="Harcourt B" first="Brian H" last="Harcourt">Brian H. Harcourt</name><name sortKey="Hickman, Carole J" sort="Hickman, Carole J" uniqKey="Hickman C" first="Carole J" last="Hickman">Carole J. Hickman</name><name sortKey="Mcgrew, Marcia" sort="Mcgrew, Marcia" uniqKey="Mcgrew M" first="Marcia" last="Mcgrew">Marcia Mcgrew</name><name sortKey="Tamin, Azaibi" sort="Tamin, Azaibi" uniqKey="Tamin A" first="Azaibi" last="Tamin">Azaibi Tamin</name></noCountry><country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Yilla, Mamadi" sort="Yilla, Mamadi" uniqKey="Yilla M" first="Mamadi" last="Yilla">Mamadi Yilla</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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